Potential interaction between presenilin and metacaspase on the Mechanism of Programed Cell Death in Leishmania infantum.
Proteases have been considered as promising targets for anti-parasitic agents, these enzymes occur in all organisms from prokaryotes to eukaryotes to viruses. The aim of the present study was to provide, through bioinformatics techniques, potential promising targets related to the apoptosis mechanism, in order to develop a vaccine and new anti-parasitic drugs.
For the identifying of the hydrophobic regions, the Kyte and Doolittle methodology was utilized. The nine hydrophobic regions identified in the presenilin, based on the physicochemical properties, suggested the occurrence of transmembrane regions that were confirmed as helices scattered in the membrane by THMM. In the metacaspase structure of L. chagasi, besides the occurrence of four hydrophobic regions, the THMM analyses predicted just one helix, placed in the N-terminal portion. The analyzes of hydrophilicity through B-EpiPred Server, indicated the occurrence of several residues localized in external regions, showing that both molecules have significant numbers of fragments with high antigenic propensity. The prediction of epitopes on the tertiary structure was obtained by the I-TASSER server.
In the present paper we are suggesting potential availability of a hybrid peptide originated from the presenilin and metacaspase of the Leishmania for the developing of new drugs or vaccine.
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